# The Regenerative Effects of Hemostatic Plant Extract in Second Degree Burn Wound

## Authors

- Erhan Cahit Ozcan ([ORCID](https://orcid.org/0000-0003-3966-7884)) — Elazig Training and Research Hospital, Department of Plastic and Reconstructive Surgery
- Sefa Senol ([ORCID](https://orcid.org/0000-0002-4150-3195)) — Elazig Training and Research Hospital, Department of Cardivascular Surgery
- Yavuz Tokgoz ([ORCID](https://orcid.org/0000-0002-4113-385X)) — Adnan Menderes University Medical Faculty, Department of Pediatrics
- Tuncay Kuloglu ([ORCID](https://orcid.org/0000-0001-9874-3838)) — Fırat University Medical Faculty Department of Histology and Embriology

## Article information

- **Journal:** The Ulutas Medical Journal (Ulutas Med J), ISSN 2149-388X
- **Issue:** Volume 3, Issue 4 (2017), pages 78–84
- **Article type:** Original Research
- **Published online:** 2017-12-27
- **DOI:** [10.5455/umj.20171108031208](https://doi.org/10.5455/umj.20171108031208)
- **Licence:** [CC BY-NC 4.0](https://creativecommons.org/licenses/by-nc/4.0/)
- **Article page:** https://www.ulutasmedicaljournal.com/?mno=282922
- **PDF:** https://www.ulutasmedicaljournal.com/pdf/282922.pdf

## Abstract

Introduction: The effects of Ankaferd Blood Stopper (ABS), a hemostatic herbal agent, on wound healing of experimental second degree thermal burn model were analyzed in this study. Material and Methods: 24 wistar albino rats were used. All rat subjects were divided into 4 equal groups as control, burn control, Ankaferd Blood Stopper and serum physiologic. In all rats except for those in negative control group, second degree thermal burn was formed with metal plates heated on their backs. Biopsies were taken on 0th,7th,14th and 21st days. In tissue samples; they were evaluated in terms of fibroblast increase histopathologically, congestion, inflammatory cell increase and epithelization findings and they were also scored. The values obtained were compared statistically. Results: No significant difference was observed between groups with regard to fibroblast increase (p>0.05). On the 7th day, congestion values in burn control group was determined higher than ABS group (p

## Keywords

Burn; burn healing; epithelization; ankaferd

## How to cite

Ozcan EC, Senol S, Tokgoz Y, Kuloglu T. The Regenerative Effects of Hemostatic Plant Extract in Second Degree Burn Wound. Ulutas Med J. 2017;3(4):78-84. doi:10.5455/umj.20171108031208

## References

1. Stephen Basil AP, Steven EW, Arthur DM. Epidemiology, demographic, and outcome characteristics of burn injury. In: Herndon DN, ed. Total Burn Care. 4thed. Galveston, TX, USA: Saunders Elsevier; 2012. p.16-45.
2. Griffs RW. Management of multiple casualties with burns. Br Med J (Clin Res Ed). 1985;5:917-8.
3. Oryan A, Alemzadeh E, Moshiri A. Burn wound healing: present concepts, treatment strategies and future directions. J wound care 2017;2;26:5-19. https://doi.org/10.12968/jowc.2017.26.1.5
4. Kahn SA, Patel JH, Lentz CW. Fire fighter Burn injures: Predictable patterns influenced by Turn out Gear. J Burn Care Res 2012;33(1):152-6.
5. Rezaei E, Beiraghi-Toosi A, Ahmadabadi A, Tavousi SH, Alipour Tabrizi A, Fotuhi K, Jabbari Nooghabi M, Manafi A, Ahmadi Moghadam S. Can Skin Allograft Occasionally Act as a Permanent Coverage in Deep Burns? A Pilot Study. World J Plast Surg 2017;6(1):94-99.
6. Bayram Y, Parlak M, Aypak C, Bayram I. Three-year review of bacteriological profile and antibiogram of burn wound isolates in Van, Turkey. Int J Med Sci 2013;10(1):19-23. https://doi.org/10.7150/ijms.4723
7. Rosenberg L, Krieger Y, Bogdanov-Berezovski A, Silberstein E, Shoham Y, Singer AJ. A novel rapid and selective enzymatic debridement agent for burn wound management: a multi-center RCT. Burns 2014;40(3):466-74. https://doi.org/10.1016/j.burns.2013.08.013
8. Arslan S, Yeşil Y, Ülger Z, Cankurtaran M, Göker H, Haznedaroğlu IC. Yaşlı Bir Hastada Yumuşak Doku Travmasına Bağlı Gelişen Yara İyileşmesinde Ankaferd. Akademik Geriartri Dergisi 2010;2(1):58-60. Regenerative Effects of ABS in Burn Wound Ozcan, et al.
9. Bahramsoltani R, Farzaei MH, Rahimi R. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review. Arch Dermatol Res 2014;306(7):601-17. https://doi.org/10.1007/s00403-014-1474-6
10. Uygun M, Yavuz OY, Uraş İ, Duman İG, Haltaş H, Kömürcü M. Effect of Ankaferd Blood Stopper on muscle healing. Clin Invest Med 2016;39(6):27526. https://doi.org/10.25011/cim.v39i6.27526
11. Goker H, Haznedaroglu IC, Ercetin S, Kirazli S, Akman U. Haemostatic actions of the folkloric medicinal plant extract Ankaferd Blood Stopper. J Int Med Res 2008;36:163‐ 1170. https://doi.org/10.1177/147323000803600121
12. İsler SC, Demircan S, Cakarer S, Cebi Z, Keskin M, Keskin C, Soluk M, Yüzbaşioğlu E. Effects of folk medicinal plant extract Ankaferd Blood Stopper on early bone healing. J Appl Oral Sci 2010;18:409-414. https://doi.org/10.1590/s1678-77572010000400015
13. Kosar A, Cipil HS, Kaya A, Uz B, Haznedaroglu IC, Goker H. The efficacy of Ankaferd Blood Stopper in antithrombotic drug induced primary and secondary hemostatic abnormalities of a rat bleeding model. Blood Coagul Fibrinolysis 2009:20;185‐ 190. https://doi.org/10.1097/mbc.0b013e32831c4cb0
14. Theoret CL. Update on wound repair. Clin Tech Equine Pract 2004;3:110-122. https://doi.org/10.1053/j.ctep.2004.08.009
15. Gauglitz GG, Korting HC, Pavicic T, Ruzicka T, Jeschke MG. Hypertrophic scarring and keloids: pathomechanisms and current and emerging treatment strategies. Mol Med 2011;17(1-2):113-25. https://doi.org/10.2119/molmed.2009.00153
16. Penn JW, Grobbelaar AO, Rolfe KJ. The role of the TGF-β family in wound healing, burns and scarring: a review. Int J Burns Trauma 2012;2(1):18-28.
17. Jimenez PA, Jimenez SE. Tissue and cellula rapproaches to wound repair. Am J Surg 2004;187:56-64. https://doi.org/10.1016/s0002-9610(03)00305-2
18. Cipil HS, Kosar A, Kaya A. Invivo hemostatic effect of the medicinal plant extract Ankaferd Blood Stopper in rats pretreated with warfarin. Clin Appl Thromb Hem 2009;15:270-276. https://doi.org/10.1177/1076029608329578
19. Huri E, Akgul T, Ayyıldız A, Ustun H, Germiyanoglu C. Hemostatik role of a folkloric medicinal plant extract in a rat partial nephrectomy model: controlled experimental trial. J Urol 2009;181:2349-54. https://doi.org/10.1016/j.juro.2009.01.016
20. Akkoc N, Akcelik M, Haznedaroglu IC. Invitro anti-bacterial activities of Ankaferd Medicinal Plant Extract. Int J Lab Hematol 2008;30:95.
21. Aktop S, Emekli- Alturfan E, Ozer C, Gonul O, Garip H, Yarat A, Goker K. Effects of Ankaferd Blood Stopper and Celox on the tissue factor activities of warfarin-treated rats. Clin Appl Thromb Hemost 2014;20(1):16-21. https://doi.org/10.1177/1076029613490254
22. Akalin C, Kuru S, Barlas AM, Kismet K, Kaptanoglu B, Demir A, Astarci HM, Ustun H, Ertas E. Beneficial effects of Ankaferd Blood Stopper on dermal wound healing: an experimental study. Int Wound J 2014;11:64–68. https://doi.org/10.1111/j.1742-481x.2012.01063.x
23. Akkoç N, Akçelik M, Haznedaroğlu İC. Göker H, Turgut M, Aksu S, Kirazlı Ş, Fırat HC. Definition of in vitro anti-fungal effects of Ankaferd medical plant extract. In: The 34th National Congress of Haematology (Proceedings Book); 8 – 11 October 2008; Çeşme, İzmir, Turkey: abstract no: S017 (in Turkish).
24. Koçak E, Akbal E, Taş A, Köklü S, Karaca G, Can M, Kösem B, Üstün H. Anti-inflammatory efficiency of Ankaferd blood stopper in experimental distal colitis model. Saudi J Gastroenterol 2013;19:126–130. https://doi.org/10.4103/1319-3767.111955
25. Göker H, Kılıç E, Uçar Çetinkaya D, Büyükaşık Y, Aksu S, Turgut M, Haznedaroğlu İC. The in vitro anticancer activity of Ankaferd on human colon cancer (CaCo-2) cells. The 10th National Congress of Internal Medicine Congress Book; 15 – 19 October 2008; Antalya, Turkey: p044 (in Turkish).
26. Uğur A, Saraç N, Çankal DA, Özle M. The antioxidant and antimutagenic activities of Ankaferd blood stopper,a natural hemostatic agent used in dentistry. Turk J Med Sci 2016;19;46(3):657-63. https://doi.org/10.3906/sag-1504-62
27. Beyazit Y, Kurt M, Kekilli M, Goker H, Haznedaroglu IC. Evaluation of hemostatic effects of Ankaferd as an alternative medicine. Altern Med Rev 2010;15:329-336.
28. Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev 2003;83:835-870. https://doi.org/10.1152/physrev.2003.83.3.835
29. Li J, Chen J, Kirsner R. Pathophysiology of acute wound healing. Clin Dermatol 2007; 25: 9-18. https://doi.org/10.1016/j.clindermatol.2006.09.007