# Protective Effects of Micro-Injected Adipose-Derived Mesenchymal Stem Cells on Ovarian Ischemia/Reperfusion Injury in Rats

## Authors

- Burcu Kasap ([ORCID](https://orcid.org/0000-0002-1768-5320)) — Muğla Sıtkı Koçman Üniversitesi
- Sukru Kasap ([ORCID](https://orcid.org/0000-0002-7885-2655)) — Muğla Sıtkı Koçman Üniversitesi
- Seda Vatansever ([ORCID](https://orcid.org/0000-0002-7415-9618)) — Department of Histology-Embryology, School of Medicine, Celal Bayar University, Manisa, Turkey.
- Remziye Kendirci ([ORCID](https://orcid.org/0000-0002-6839-8823)) — Department of Histology-Embryology, School of Medicine, Celal Bayar University, Manisa, Turkey.
- Osman Yilmaz ([ORCID](https://orcid.org/0000-0001-7512-5076)) — Department of Laboratory Animal Science, School of Medicine, Dokuz Eylul University, İzmir, Turkey.
- Meryem Calisir ([ORCID](https://orcid.org/0000-0002-9890-8943)) — Department of Laboratory Animal Science, School of Medicine, Dokuz Eylul University, İzmir, Turkey.
- Ummuhani Ozel Turkcu ([ORCID](https://orcid.org/0000-0003-2244-7965)) — Department of Clinical Biochemistry, School of Medicine, Mugla Sitki Kocman University, Mugla, 48000, Turkey
- Melike Nur Akin ([ORCID](https://orcid.org/0000-0001-6794-846X)) — Department of Obstetrics and Gynecology, School of Medicine, Mugla Sitki Kocman University, Mugla, 48000, Turkey
- Eren Akbaba ([ORCID](https://orcid.org/0000-0002-4724-0779)) — Department of Obstetrics and Gynecology, School of Medicine, Mugla Sitki Kocman University, Mugla, 48000, Turkey
- Nilgun Ozturk Turhan ([ORCID](https://orcid.org/0000-0002-9084-0630)) — Department of Obstetrics and Gynecology, School of Medicine, Mugla Sitki Kocman University, Mugla, 48000, Turkey

## Article information

- **Journal:** The Ulutas Medical Journal (Ulutas Med J), ISSN 2149-388X
- **Issue:** Volume 7, Issue 4 (2021), pages 237–247
- **Article type:** Original Article
- **Published online:** 2021-12-26
- **DOI:** [10.5455/umj.20211019101154](https://doi.org/10.5455/umj.20211019101154)
- **Licence:** [CC BY-NC 4.0](https://creativecommons.org/licenses/by-nc/4.0/)
- **Article page:** https://www.ulutasmedicaljournal.com/?mno=134160
- **PDF:** https://www.ulutasmedicaljournal.com/pdf/134160.pdf

## Abstract

Introduction: The authors investigated the immunohistochemical, histological, and biochemical effects of adipose-derived stem cells (ADSC) which possess antioxidant and anti-inflammatory properties in the ovarian ischemia/ reperfusion rat model. Materials and Methods: Rats were randomly allocated to sham, ADSC, torsion/detorsion, torsion/detorsion/ADSC groups. ADSCs were micro-injected to torsion/detorsion/ADSC during the detorsion procedure after a 6-h period of torsion. BrdU, caspase-3, TUNEL, eNOS, and iNOS staining were used. Advanced oxidation protein products (AOPPs), superoxide dismutase (SOD) activity, and levels of total sulfhydryl (TSH) were evaluated. Results: Serum AOPP increased in the torsion/detorsion, tissue and serum AOPP levels in the torsion/detorsion/ ADSC group were decreased. Tissue SOD activity in torsion/detorsion and torsion/detorsion/ADSC groups was significantly decreased compared to the sham group. The iNOS was stronger in the torsion/detorsion group following torsion and detorsion but decreased after ADSC microinjection in the torsion/detorsion/ADSC group. ADSC treatment after torsion-detorsion also reduced caspase-3 immunoreactivity and apoptotic cell number. Conclusion: ADSC implementation after torsion/detorsion might protect the ovary from ischemia/reperfusion injury.

## Keywords

Adipose-derived stem cells; Ischemia; Ovary; Rat; Torsion

## How to cite

Kasap B, Kasap S, Vatansever S, Kendirci R, Yilmaz O, Calisir M, et al. Protective Effects of Micro-Injected Adipose-Derived Mesenchymal Stem Cells on Ovarian Ischemia/Reperfusion Injury in Rats. Ulutas Med J. 2021;7(4):237-247. doi:10.5455/umj.20211019101154

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